[ Page 04 ] Chemistry & Bioactives

A whole-strain probiotic system, not one isolated compound.

Bacospore® is a microbial ingredient, not a botanical extract. Its active biological component is a viable, spore-forming strain of H. coagulans MTCC 25308, standardized to total viable spore count and associated with five functional bioactive groups.

01 · The active biological system

Standardized to viable spores, not a marker molecule.

Unlike extracts standardized to a percentage of one chemical marker, Bacospore® is standardized according to its total viable spore count. Bacospore® 300B contains not less than 300 billion viable spores per gram, determined via a pour-plate microbiological method.

The strain also produces or is associated with multiple functional metabolites: organic acids, antimicrobial substances, exopolysaccharides and digestive enzymes. Together these form a broader probiotic system rather than a single isolated active.

02 · Bioactive groups

Five functional bioactive groups.

01

Viable H. coagulans spores

Spore-forming probiotic microorganism

Provides stability during storage and supports survival through acidic gastrointestinal conditions.

Spores are identified microscopically as small, oval, retractile bodies located near the ends of the vegetative cells. They resist heat and acidic environments.

02

Lactic acid & short-chain organic acids

Organic acids · lactic acid (C₃H₆O₃)

Contributes to acidification of the local microbial environment; may help restrict undesirable microorganisms.

Bacospore® is a lactic acid-producing microbial preparation, and short-chain organic acids are among the strain's functional components.

03

Bacteriocin-like antimicrobial substances

Antimicrobial peptides / peptide-like metabolites

Supports microbial balance by producing substances capable of restricting competing organisms.

Bacteriocin-like antimicrobial substances are part of the bioactive profile of B. coagulans and contribute to its competitive activity in the gut.

04

Exopolysaccharides

Microbial polysaccharides · variable polymers

Extracellular metabolite class associated with probiotic activity and microorganism–environment interactions.

Exopolysaccharides are among the bioactive components produced by B. coagulans, contributing to how the strain interacts with the intestinal environment.

05

Digestive enzymes

Amylases and proteases

Supports carbohydrate and protein breakdown; may improve digestion and nutrient availability.

Amylases and proteases are produced by the strain and are associated with improved nutrient digestion and absorption.

03 · Why a whole-strain probiotic

Complete strain-level system, not a single molecule.

The activity of Bacospore® depends on viable spores, germination capacity, microbial metabolism and the production of multiple enzymes and metabolites. Isolating only lactic acid, one enzyme or one antimicrobial peptide would not reproduce the complete biology of H. coagulans MTCC 25308, which is why research evaluates the strain or its culture preparation as a whole.

04 · Standardization

Analytical methods & physicochemical properties.

Potency
≥ 300 × 10⁹
viable spores / gram (Bacospore® 300B)
Assay method
Pour-plate viable spore count
Identity
Microscopy · terminal oval spores
Loss on drying
≤ 5.0%
Batch consistency
Viable count, identity, moisture, microbial purity, heavy metals

Potency is determined by pour-plate viable spore count, the method that matches a living probiotic rather than a small-molecule marker.

AppearanceLight brown to dark brown powder
OdourCharacteristic
SolubilitySoluble in water
CarrierTapioca-derived maltodextrin
Manufacturing processControlled fermentation
Shelf lifeTwo years
StorageBelow 25°C in a cool, dry place, tightly sealed
ProcessingNon-irradiated · not treated with ETO
GMO statusGMO-free

Evaluate Bacospore® in your next formulation.